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Transient Transfection
Mammallian Cells
Mammalian transient expression systems are very widely used for many applications including early-stage development of biopharmaceutical protein drugs and vaccines, in vivo toxicity experiments, diagnostic testing, and many others. With CHO and HEK293 suspension cells serving as expression hosts for our Mammalian Transient Transfection Platform, our customers can expect complete, stable, and efficient production of protein ranging from milligrams to as much as grams.
Platform Advantages
More Efficient
- Transfection process is streamlined and efficient. Protein expression can be detected as early as 2 - 3 days after transfection.
More Capable
- Using our proprietary media formulations in combination with transfection reagents and specialized transfection equipment, KCell Biosciences maximizes performance advantages of this platform.
More Cost-effective
- By utilizing our platform and services, customers can save total cost associated with expensive equipment, consumables, plasmid preparation and culture time, etc. Outsourcing for production of your protein can dramatically reduce production cost.
Platform of mammalian cell transient transfection
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| CHO Transient Transfection System | HEK293 Transient Transfection System | HEK293 Transient Transfection System | |
|---|---|---|---|
| Cell Type | CHO cells | 293 cells | 293 cells |
| Growth Medium | CD CHO Transfection Media | CD 293 Medium | HEK293 SFM 616 |
| Transfection Reagent | Electroporation Buffer | Transfection Helper | Electroporation Buffer |
| Growth Medium post Transfection | CD CHO Transfection Media | CD 293 Transfection Medium | HEK293 SFM 616 |
| Expression Medium post Transfection | CD CHO Transfection Feed | HEK293 Transfection Feed | HEK293 Transfection Feed |
| Expression Enhancer | Feed B (100x) | Feed B (100x) | Feed B (100x) |
| Suggested Transfection Cell Density | 5.0 * 106 cells/mL | 3.0 * 106 cells/mL | 3.0 - 6.0 * 106 cells/mL |
| Transfection Method | Electroporation | Chemical transfection | Electroporation |
| Medium Volume | 40 - 2,000 mL | 40 - 2,000 mL | 40 - 2,000 mL |
| Titer | >3.0 g/L | 1.0 g/L | 1.0 g/L |
Service Content
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| Description | Experimental content | Delivery |
|---|---|---|
| Vector Construction | Option 1 : Clients provide an amino acid sequence or gene sequence, then KCell Biosciences clones it into an expression plasmid. Option 2 : Clients provide an established vector. |
Sequencing report Lab report |
| Transient Transfection | Proliferation and passaging of host cells without vector ▶ Transient Transfection ▶ Fed-batch expression ▶ product harvest and test (Choose from HPLC, ELISA, and Fortebio) |
Production and yield report Supernatant |
| Protein purification (optional) |
Protein A one-step purification (proceed to SEC-HPLC, SDS-PAGE or WB based on need) |
Protein purification, Purification experiments, and protein Detection report |
Delivery time
Samples shipped within 2 - 3 weeks after the contract is signed
Service Flow Chart
